Lung tumor spheroids for onco-immunological research

03:092 years ago

Lung cancer thrives in a complex multicellular tumor microenvironment that impacts tumor growth, metastasis, response, and resistance to therapy. While orthotopic murine lung cancer models can partly recapitulate this complexity, they do not resonate with high-throughput immunotherapeutic drug screening assays. To address the current need for relevant and easy-to-use lung tumor models, we established a protocol for fully histo-compatible murine and human lung tumor spheroids, generated by co-culturing lung fibroblasts with tumor cells in ultra-low adherence 96-well plates. Moreover, we describe their application potential to study tumor-stroma organization, T-cell motility, and infiltration as well as distinct macrophage subsets’ behavior using confocal microscopy. Finally, we report on a 3D target specific T-cell killing assay that allows spatio-temporal assessment using live cell imaging and flow cytometry. This lung tumor spheroid platform can serve as a blueprint for other solid cancer types to comply with the need for straightforward onco-immunology assays.

Related

EURL ECVAM
Projects and initiatives
HealthInnovationPolicy

EURL ECVAM

The EU Reference Laboratory for alternatives to animal testing (EURL ECVAM) promotes and facilitates the use of non-animal methods in testing and research. It validates, disseminates and shares knowledge on the 3Rs (Replacement, Reduction and Refinement of animal experiments). In this video, Raffaella Corvi explains what EURL ECVAM does in the field of safety testing of chemicals while reducing laboratory animal testing. Watch the accessible version of the video here (https://audiovisual.ec.europa.eu/en/video/I-230374). ©European Union, 2021
02:335 months ago
Five simple tricks for making your own video for TPI.tv
TPI.tv videos

Five simple tricks for making your own video for TPI.tv

This video shows you how to make a video yourself. It's really not that difficult! See also the submission page (https://tpi.tv/submit-a-video) for additional information.
01:234 years ago
 Helpathon #11 – Can you help Terry?
Questions
HelpathonsHealth

Helpathon #11 – Can you help Terry?

Terry Vrijenhoek (UMC Utrecht) is a geneticist and explores the societal impact of gene therapy. In this Helpathon the focus is on Alzheimers, for which there is no cure but there is a promising RNA-based therapy in the pipeline. Can you help Terry with designing scenarios for responsible development for gene therapy for Alzheimer disease in terms of benefits, risks, budgets and animal models? More information can be found here (https://www.helpathonhotel.org/coming-up).
01:142 months ago
Helpathon #11 – Can you help Francesca?
Questions
HelpathonsHealth

Helpathon #11 – Can you help Francesca?

Francesca Stillitano (UMC Utrecht) is a geneticist and an Assistant Professor at the Department of Cardiology. Francesca is currently working with mice models and with human tissue-based in vitro models to develop and test new gene therapies for a rare cardiomyopathy. Can you help Francesca with developing gene therapies for curing inherited cardiomyopathies without the use of animal models? More information can be found here (https://www.helpathonhotel.org/coming-up).
01:552 months ago